3 Stages of Interphase

By Joshua Bush
Interphase is part of the normal cell cycle.

The main stages of the cell cycle are interphase, mitosis and cytokinesis. Mitosis and cytokinesis are the stages when duplicated contents are physically separating into two separate cells. During the activities of interphase, the cell is monitoring its environment and preparing itself for the next division. Interphase activity is further broken down into three stages: G1, S and G2. Cells spend most of their lifespan in the interphase stages.

G1 Phase

The cell cycle begins with G1, or first gap, which is the time between birth of a new cell and the beginning of DNA replication. During G1, the cell grows in size and begins protein synthesis and organelle duplication. The cell is also monitoring its environment to ensure that external conditions are suitable for cell division. The first internal checkpoint on cell division also occurs in G1, making sure that the DNA is not damaged before proceeding to the DNA duplication step.

S Phase

After passing through the first internal checkpoint, the cell enters the S phase, or synthesis phase. During the S phase, DNA is duplicated so that each chromosome contains two identical copies. These DNA copies do not separate into duplicate chromosomes until after the interphase stages end and mitosis begins. The S phase ends when the total amount of DNA has doubled and the cell enters the G2 phase.

G2 Phase

During the second gap, or G2 phase, the cell continues to grow in size and produce proteins necessary for cell division. Microtubules necessary for separating the chromosome copies are made during this phase. The second internal checkpoint that determines if the cell can continue through its cycle occurs in G2. The G1 checkpoint checks to make sure the DNA is error free before replication, and this checkpoint in G2 makes sure the new DNA is error free after replication.

G0 Phase

Cells can also pause interphase during G1 and enter a resting state called G0. If the conditions outside the cell do not support division, the cell can pause in G0 for an indeterminate amount of time before resuming the cell cycle and normal growth. If conditions change, the cell can either move from G0 back into the G1 phase, or become post-mitotic and leave the cell cycle completely.

About the Author

Joshua Bush has been writing from Charlottesville, Va., since 2006, specializing in science and culture. He has authored several articles in peer-reviewed science journals in the field of tissue engineering. Bush holds a Ph.D. in chemical engineering from Texas A&M University.