The Types of Electrophoresis

By Amelia McDoogleburger; Updated April 24, 2017
SDS-PAGE electrophoresis gel

Gel electrophoresis is commonly used as an identification and analysis tool; various methods are used to separate DNA, RNA and proteins.

Agarose Gel Electrophoresis

Agarose gels are commonly used to sort DNA and RNA molecules based on size. The agarose gel concentration can be varied, based on the size of the molecules that need to be isolated.

SDS-PAGE Electrophoresis

Sodium dodecyl sulfate - polyacrylamide gel electrophoresis is used to separate proteins based on size. The proteins are unfolded, or denatured, using SDS detergent, and run on a polyacrylamide gel.

DNA Sequencing Gels

Denatured DNA can be run on polyacrylamide gels, which allows scientists to determine the sequence of the molecule.

Native Protein Electrophoresis

Proteins can remain folded in the native conformation and run on gels to separate them by both mass and charge.

Electrofocusing Electrophoresis

Electrofocusing separates proteins on the basis of charge as well as pH; the gel used in this type of electrophoresis has a pH gradient.

About the Author

Amelia McDoogleburger loves science and has been a biology tutor for over five years. Her first professional publication was in 2001 in the "Journal of Cerebral Blood Flow and Metabolism," and she has also been published in the "Journal of Cell Biology." Amelia holds a Bachelor of Science degree in biology, computer science and mathematics and a Doctor of Philosophy in biochemistry.