Compound light microscopes are valuable tools in the lab. They magnify our ability to see in detail by up to 1,000 times, allowing us to study things as small as the nucleus of a cell. With them, we can determine the shape and structure of cells, observe the movements of microorganisms, and examine the smallest parts of plants, animals and fungi. Because the objects under a microscope’s view are so small, it is often impossible to use a ruler to determine their size. However, calculating a microscope’s field of view (FOV), the size of the area visible through the microscope, allows you to determine the approximate size of a specimen under examination.
TL;DR (Too Long; Didn't Read)
Examine Your Microscope
Calculating Field of View
Changing Magnification and Measurement
To determine the FOV of your microscope, first examine the microscope itself. The microscope’s eyepiece should be labeled with a sequence of numbers, such as 10x/22 or 30x/18. These numbers are the eyepiece magnification and the field number, respectively. Also, take note of the magnification of your objective lens at the bottom of the microscope, if applicable – generally 4, 10, 40 or 100 times.
Once you’ve taken note of the eyepiece magnification, field number and objective lens magnification number, if applicable, you can calculate your microscope’s field of view by dividing the field number by the magnification number. For example, if the microscope’s eyepiece reads 30x/18, then 18 ÷ 30 = 0.6, or an FOV diameter of 0.6 millimeters. If your microscope only uses an eyepiece, this is all you need to do, but if your microscope uses both an eyepiece and an objective lens, multiply the eyepiece magnification by the objective magnification to find the total magnification before dividing the field number. For example, if the eyepiece reads 10x/18, and the magnification of your objective lens is 40, multiply 10 and 40 to get 400. Then divide 18 by 400 to get an FOV diameter of 0.045 millimeters.
Whenever you change microscopes or switch eyepieces or objective lenses, remember to repeat the FOV calculations with the new field number and magnifications. When dealing with objects observed at higher magnifications, it may be useful to convert your measurements from millimeters to micrometers. To do so, multiply the FOV diameter in millimeters by 1,000 to convert the diameter to micrometers.