Colony Characteristics of E.Coli

Colony Characteristics of E.Coli
••• anyaivanova/iStock/GettyImages

Escherichia coli (commonly known as E. coli) are a large, varied group of bacteria found in the environment, foods and lower intestines of humans and animals. Most strains of E. coli are harmless, but some strains cause food poisoning in humans. A bacterial colony is a visible mass of microbial cells originating from one single cell. You can identify E. coli if you know its colony characteristics.

TL;DR (Too Long; Didn't Read)

E. coli colonies are off-white, dry in texture, with a steady growth pattern. E. coli colonies have no pigment, but change color when transformed by a plasmid.

Arrangement and Size

The arrangement and size of an E. coli colony are not reliable characteristics. The arrangement – how close together or far apart the bacteria are is due to how they were streaked on the plate. Colony size can vary because a colony is larger if it's more spread out. Color, texture and growth pattern are better colony characteristics to observe.

Color, Texture and Growth Pattern

An E. coli colony is off-white or beige in color with a shiny texture. It often looks like mucus or a cloudy film over the whole surface of the plate. An E. coli colony is slightly raised and has an entire, fixed margin and a steady growth pattern, creating concentric growth rings in the colony. You can detect these rings under a microscope. Older colonies often have a darker center.

E. Coli Cells

Bacteria are unicellular microorganisms with no chlorophyll pigments. There are no nucleus or membrane-bound organelles, so the cell structure is simpler than that of other organisms. All bacteria have a rigid cell wall, meaning they maintain a definite cell shape. E. coli cells are bacillus (rod-shaped) and typically occur individually and in large clumps. The average size of the rod is 1.1 to 1.5 µm wide by 2.0 to 6.0 µm long.

Transforming E. Coli

Unlike some other bacteria, E. coli have no pigment, so their colonies are lacking in color. However, you can give E. coli a plasmid (a small, circular strand of DNA) that includes a color marker to transform it. Successful E. coli transformations create colored colonies on some media. For example, growth on a MacConkey agar plate shows that E. coli are not inhibited by bile salts and crystal violet. The pink color of the bacterial growth indicates E. coli can ferment lactose and tells you that it is a gram-negative bacterium.

Related Articles

Characteristics of Salmonella Bacteria
What Is Arrangement in Microbiology?
How Is a Pure Culture Prepared Directly?
How to Identify an Unknown Bacteria in Microbiology
How to Stain Chitin
How to Identify Mold in Petri Dishes
How to Count Colonies in Microbiology
What Types of Bacteria Produce Endospores?
How Enterococcus Faecalis Changes the Mannitol Salt...
What Is a CFU in Microbiology?
What Types of Cells Are Bacteria?
What is a Subculture in Microbiology?
How to Calculate the Amount of Bacteria Present
Technique to Separate Bacteria in a Mixed Culture
Types of Agar Plates
Isolation Techniques for a Streak Plate
What Is an Example of a Colony in Microbiology?
Types of Coccus Bacteria
The Advantages of Stained Bacteria
What Is a Tetrad in Microbiology?