How to Calculate Kovats Index

Kovats index is used in chromatography, which is performed in common services such as forensics, drug testing and airport security scanning.
••• Creatas/Creatas/Getty Images

Chromatography is the science of decomposing elements into separate compounds that can be identified and analyzed. Gas chromatography achieves this process by subjecting the substances to high degrees of heat so the gaseous components can be separated. Gas chromatography is one of the primary techniques used in forensics science, transportation security scans and drug testing labs. This procedure is one of the first tests performed after a sample has been separated into components, and it is used to identify each compound drawn from the sample. Kovats retention index is a mathematical tool used to assist researchers in identifying the compound and ascertaining a degree confidence in this discovery.

    Analyze the components. The content of each component is analyzed using three techniques: qualitative analysis (numeric assessment of known species), retention time (comparison of unknown species) and Kovats retention index (logarithmic scale on which adjusted retention times are compared with unbranched alkanes).

    Identify the formula. Kovats index(I) is calculated as I= 100[n + (N - n) x (Log tr (unknown) - logtr (n))/ logtr(N) - logtr(n)) where n equals the number of carbon atoms in the smaller alkane, N is the number of carbon atoms of the larger alkane, tr is in all cases the adjusted retention time (calculated at time - time of the undelayed small compound).

    Compute Kovats index. Given the complexity of this procedure, it is best to utilize existing software system designed to compute the indices automatically. Dionex, Chrom Perfect and Agilent Technologies offer software packages that support gas chromatography and provides the appropriate indices for analysis.

Related Articles

How to Calculate the Degree of Polymerization
How to Calculate a Distillation System
Spectrometer Experiments
How to Calculate HPLC Resolutions
How to Calculate a T-Statistic
Endpoint Math Formula
How to Calculate a Semivariogram
How to Find The Slope of a Line Given Two Points
How to Convert Rockwell Hardness to Tensile Strength
How to Calculate the Refractive Index of a Formulation
How to Convert Micromolar to PPM
How to Solve for Volume
Advantages & Disadvantages of XRD and XRF
How to Make a Bacterial Flowchart
How to Calculate the Empirical Formula
How to Find a Z Score
How to Calculate the Calculations for Spectrophotometers
How to Plot a Lognormal Curve
How to Calculate the Degree of Unsaturation

Dont Go!

We Have More Great Sciencing Articles!