Bromothymol blue solution is used as an indicator to determine the rough pH of a substance. It is prepared from a powder, household items and common laboratory chemicals that can be obtained through a scientific supply house individually or as a kit. The mixed bromothymol blue solution will turn yellow in an acidic solution and blue in basic solutions.
- 0.1 g bromothymol blue powder
- 10 mL 4 percent sodium hydroxide solution
- 20 mL rubbing alcohol
- 1 L distilled water
- Stirring rod
- Safety goggles
- Rubber gloves
Sodium hydroxide is a corrosive chemical. Be sure to wear all safety gear, including gloves and goggles. Bromothymol blue should be prepared with scientific grade equipment in a laboratory environment.
Add 0.1 g of bromothymol blue powder to 10 mL of 4 percent sodium hydroxide solution in a large beaker.
Combine 20 mL of alcohol to the sodium hydroxide/bromothymol blue solution.
Dilute the mixture with 1 L of distilled water in a large container. The solution should be dark blue in color.
If the solution appears green, use a pipette to slowly add sodium hydroxide drop by drop to the solution until it changes to blue.
Things You'll Need
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