How to Make Forensic Luminol

Hydrogen peroxide is one ingredient in luminol.
••• brown bottle image by Jim Mills from

You are probably familiar with forensic luminol from the many references to it on TV crime shows. It is sprayed on areas where blood is believed to be present. The luminol reacts with the iron in blood hemoglobin and glows a bluish purple when the lights are turned out. It will actually react to any iron present on a surface where it is sprayed. (Ref 1) You can buy luminol, but you can also make it on your own.

    Mix the luminol powder and washing soda together as a dry mixture in a bowl.

    Stir in the distilled water a little at a time.

    Add the hydrogen peroxide to the mixture in the bowl a little at a time. Stir the solution thoroughly.

    Pour the solution into a spray bottle.

    Spray the luminol on a bloodstained cloth and turn out the lights. The cloth should glow a bluish-purple.

    Things You'll Need

    • Bowl
    • Spoon
    • 0.2g luminol powder
    • 10g washing soda
    • 180mL distilled water
    • 180mL 3% hydrogen peroxide
    • Spray bottle
    • Bloodstained cloth


    • This solution is not very stable and changes to a gaseous state within hours. Store the luminol in a tightly sealed bottle if you plan to use it again.

Related Articles

How to Make Sodium Nitrate
Chemicals Used in Forensic Science
How to Make Homemade Glow Sticks
How to Test UV Bulbs
What Is Pumice Powder Used For?
Why is Quinine Fluorescent?
Test Your Knowledge on Middle School Science
List of Household Bases & Acids
How to Make Phosphorus
How to Remove Iron Filings From Magnets
How to Extract Iodine From Potassium Iodide
Why Do Avocados Turn Red?
How to Mix Ammonia with Glycerine
How to Test for Hydrochloric Acid
How to Tell a Synthetic Pink Tourmaline
Does Oil Dissolve Rubber Gloves?
How to Detect Potassium Nitrate
How to Identify Obsidian
How to Make Ozone Test Strips

Dont Go!

We Have More Great Sciencing Articles!