How to Make Your Own Microscope Stain

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Amateur microscopy is a low-cost way for students and science enthusiasts to observe the world in miniature. With an inexpensive consumer-grade compound microscope and a handful of cheap slides, you can embark on a journey of scientific exploration that begins in your own backyard. Stain your specimens to increase contrast and exhibit more detail under the microscope. Though commercial stains are available for purchase from scientific supply stores, you may make your own microscope stains at home using easily obtained dyes and a scientific process of trial and error.

    Choose your dye. Common household dyes include red or blue food coloring, iodine or India ink. You may also obtain methylene blue or malachite green dye from the aquarium section of a pet supply store.

    Dilute the dye with distilled water. The optimal dilution rate will vary depending on the strength and type of your dye, as well as the object to be stained. To begin, use a 1:1 dilution ratio of dye solution to water.

    Mount your specimen on a glass slide. For a simple wet mount, apply a drop of water to the slide and carefully place the specimen on the wet portion.

    Apply a few drops of diluted dye to the sample. Keep the dye and the samples in contact for one to three hours to allow time for dye absorption. Rinse the dye from the sample using an eyedropper full of distilled water. Place the coverslip on top and examine the slide under the microscope.

    Adjust the dye formulation through trial and error. If the dyeing is unsatisfactory, add a few drops of vinegar to the solution to make the pH more acidic. Experiment with higher concentrations of dye. Use multiple dyes in the same solution to achieve a different staining effect.

    Tips

    • Homemade dyes also work with more complicated mounting techniques, such as fixatives and dry mounts. Mount your slides according to your usual process, substituting the homemade solution for your normal dye.

References

About the Author

Mary MacIntosh has been writing professionally since 2007, contributing articles to "The California Tech" and serving as an editor for the "Biweekly Frink Digest." She is pursuing a Bachelor of Science in computational neuroscience at the California Institute of Technology.

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