The Methods of Enumeration in Microbes

By Karl Wallulis

The methods of enumeration in microbes can be divided into four categories. Direct methods involve counting the microbes, while indirect methods involve estimation. Viable methods only count cells that are metabolically active, while total counts include dead and inactive cells.

Direct/Viable

A direct/viable method involves a standard plate count, in which repeated dilutions of a sample are counted to calculate the count in the original sample.

Indirect/Viable

Indirect/viable methods such as MPN (most probable number) involve making a statistical inference about the microbe count based on patterns of growth.

Direct/Total

The microbes are counted with the aid of fluorescent stains and dyes, which make the microbes visible with the aid of a fluorescent microscope.

Indirect/Total

Spectroscopy is a form of indirect/total enumeration, which involves estimating the amount of microbes based on the amount of light passed through the culture by a spectrophotometer.

About the Author

Karl Wallulis has been writing since 2010. He has written for the Guide to Online Schools website, covering academic and professional topics for young adults looking at higher-education opportunities. Wallulis holds a Bachelor of Arts in psychology from Whitman College.